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Fig. 1 | Gut Pathogens

Fig. 1

From: In vivo animal models confirm an increased virulence potential and pathogenicity of the NAP1/RT027/ST01 genotype within the Clostridium difficile MLST Clade 2

Fig. 1

Effect of bacterial cell-free supernatants of Clade 2 C. difficile strains in the ligated murine ileal loop. Bacteria-free supernatants from the indicated genotypes were prepared in TYT-broth. 7–10 mice per group were inoculated with 0.3 ml of supernatant in ligated ileal loops. Four hours after inoculation, mice were euthanized and data was recorded. a ratio weight/length, bars represent means of the normalized weight of ileal sections. b myeloperoxidase activity (MPO) for each group. Cytokines levels were measured on intestinal tissue. c TNF-α, d IL-1β, and e IL-6. *p <0.05 One-way ANOVA followed by Bonferroni’s correction. The mean of measurements obtained for control groups (PBS, TYT-broth and non-toxigenic supernatant) is indicated by a dashed line

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Gut Pathogens

ISSN: 1757-4749