Fig. 5

miR-212-5p regulates the apoptosis of hepatocytes induced by Hcy via targeting PSMD10. A Schematic representation of binding sites of miR-212-5p in the PSMD10 3′UTR analyzed by TargetScan database (http://www.targetscan.org/vert_72/). B Luciferase activity of pMIR-PSMD10-wt or pMIR-PSMD10-mut reporters in the presence of miR-212-5p mimics or negative control (NC) (n = 3). C, D The levels of miR-212-5p in cbs^+/− mice livers and the hepatocytes treated with 100 μmol/L Hcy were detected by qRT-PCR and normalized by U6. E, F PSMD10 expression was measured by qRT-PCR and western blot in hepatocytes after transfection with miR-212-5p mimics, miR-212-5p inhibitor or miR-neg control. G, H The expression levels of GRP78, p-PERK, PERK, p-eIF2α, eIF2α, CHOP, ATF6, cleaved caspase-3, cleaved caspase-12, Bcl-2 and Bax were detected by western blot in hepatocytes transfected with miR-212-5p mimic and treated with 100 μmol/L Hcy. I The apoptosis ratio of hepatocytes was analyzed by flow cytometry analyses after the cells were transfected with miR-212-5p mimic and treated with 100 μmol/L Hcy. All data are expressed as mean ± SD. ^*P < 0.05, ^**P < 0.01 versus cbs^+/+, control, mic-NC or inh-NC group ^#P < 0.05, ^##P < 0.01 versus mic-NC + homocysteine or inh-NC + homocysteine group.