Fig. 2

Identification of E. coli serotypes O22:H8 and O91:H21 in mouse feces. A E. coli in mouse feces was isolated by Violet Red Bile Lactose agar (VRVL). B PCR analysis confirmed that the bacteria isolated from the mouse feces were E. coli. C 16S rRNA sequencing for E. coli. E. coli-Type II showed differences in nucleotide positions 52, 113, 116, 136, 263, 293, and 424 of the 16S rRNA sequence from E. coli-Type I. D–I Whole genome sequencing of E. coli showing relative abundance heatmaps of genes in the serotypes O22:H8 and O91:H21. D products. E virulence factors. F ECNumber: Enzyme Commission number, the Enzyme nomenclature database. G Serofinder H: A FASTA database of specific O-antigen processing system genes for O typing and flagellin genes for H typing publicly available Web tools hosted by the Center for Genomic Epidemiology (CGE). H Abricate: a database for mass screening of contigs for antimicrobial resistance or virulence genes bundled with multiple databases: NCBI, CARD, ARG-ANNOT, Resfinder, MEGARES, EcOH, PlasmidFinder, E. coli_VF and VFDB. I. Napdos: a bioinformatic tool for the rapid detection and analysis of secondary metabolite genes. This tool is designed to detect and extract C- and KS- domains from DNA or amino acid sequence data, including PCR amplicon products, individual genes, whole genomes, and metagenomic data sets. J. PCR analysis showing higher levels of Long Polar Fimbriae Type I (LpfA) contained in E. coli O91:H21