Fig. 5

Fpr2 deficiency delayed the recovery of the damaged colon mucosa in mice with colitis. A Reduced survival of Fpr2^−/− mice after administration of 3% DSS in drinking water for 5 days followed by an additional 7 days of normal water intake. n = 12 mice/group. B Increased damage for colon mucosa in Fpr2^−/− mice treated with DSS for 5 days. Scale bar = 50 µm. Right: Quantitation of crypt damage in Fpr2^−/− mice after DSS treatment. n = 6 mice per group. **P < 0.01. C Reduced number of Ki67⁺ cells in the colon mucosa of Fpr2^−/− mice after administration of 3% DSS in drinking water for 3 days followed by an additional 4 days of normal water intake. Scale bar = 30 µm. Right: Quantitation of Ki67⁺ cells per crypt, n = 30 crypts from 4 mice/group. ****P < 0.0001. D Reduced number of goblet cells in the colon of Fpr2^-/- mice. An alcian blue stain. Scale bar = 50 μm. Right panel: Quantitation of PAS+ goblet cell number in the colon, n = 29-35 fields from 4 mice/group. ****P < 0.0001. F Increased production of IL-1β in the colon mucosa of Fpr2^−/− mice after administration of 3% DSS in drinking water for 3 days followed by an additional 4 days of normal water intake. Red: IL-1β, Blue: DAPI. Scale bar = 30 μm. Right: Quantitative IL-1β + fluorescence intensity, n = 30 crypts from 4 mice/group. ****P < 0.0001