Fig. 2

m-NGS A and mass spectrometry B analyses of abdominal biopsy tissue indicated C. koseri infection. A: The C. koseri genome sequences mapping by m-NGS. The result of mNGS showed 263387 reads corresponding to the C. koseri, with a coverage of 88.1636%. The X-axis represents the genome size of C. koseri and the Y-axis represents the number of sequences detected within different genomic segments. B: Mass spectrum of C. koseri. MALDI-TOF mass spectrometry showed the specific spectrum, which matched the registered pattern of C. koseri. The X-axis represents the mass-to-charge ratio (m/z) of different peptide fragment ions and the Y-axis represents the ion intensity. Peaks at specific m/z values indicate the presence of corresponding ions in the sample. The intensity of each peak reflects the abundance of the corresponding ion. m-NGS metagenomic next-generation sequencing, MALDI-TOF mass spectrometry matrix-assisted laser desorption ionization time-of-flight mass spectrometry