Fig. 3

Relative gene expression of relevant miRNAs and their target mRNAs in secondary abiotic IL10 ^−/− mice colonic tissue sections six days post C. jejuni infection. 19 C. jejuni infected and 20 naïve control mice were included in the study. Each data point in the diagram represents an animal that showed confirmed expression in the respective analysis. A Upon C. jejuni infection, expression of miR-125a-5p was significantly decreased, whereas transcriptional levels of St3gal1 and B4galt1 were significantly enhanced. B miR-615-3p levels were significantly increased, while St3gal2 was significantly downregulated. Expressions were relatively calculated to naïve controls and normalised with SNORD44 and SNORD47 or with HPRT and SDHA, respectively. For each individual, the mean was calculated based on triplicate measurements. Charts show mean ± SD of all individuals. Statistical significance is presented by asterisks compared to naïve controls. *P ≤ 0.05, ***P ≤ 0.001, ****P ≤ 0.0001, unpaired t-test