Fig. 4

Increased colonic ST3GAL1 protein levels in secondary abiotic IL10 ^−/− mice six days post C. jejuni infection compared to naïve controls. A Increased ST3GAL1 protein levels were detected by western blots in pooled protein samples of infected mice. GAPDH is presented as the respective loading reference. B ST3GAL1 detection of eight C. jejuni infected and eight naïve mice colon samples were quantified by means of western blotting followed by densitometric analysis relative to the respective GAPDH signals as controls. Protein levels of ST3GAL1 in the infected group were significantly and 1.7-fold increased, compared to naïve controls. Charts show normalised mean ± SD in each group. Statistical significance is presented by asterisks. **P ≤ 0.01, unpaired t-test. C Localisation and enhanced ST3GAL1 expression in the colon of C. jejuni infected mice determined in representative immunofluorescent staining compared to naïve controls. ST3GAL1 was detected by immunofluorescent staining and shown in red using DyLight 594 whereas the nuclei were stained blue using DAPI. Enhanced red signal intensity and pronounced cytosolic distribution was detected in infected controls. The top row shows the overview, with the area outlined in green in the bottom row shown enlarged. Scale bars indicate 100 µm and 50 µm. Exposure time was identical for all colon sections and presented images are representative for three biological replicates tested