Table 2 List of the primers used for cloning and gene expression in this study
Target gene | Purpose | Primer sequence | Amplicon size (bp) | Annealing temperature (°C) | Source |
|---|---|---|---|---|---|
MuIFNλ3 | Cloning into pHis-TEV Plasmid | F-5′ATAGGATCCATGCTCCTCCTGCTG 3′ R-5′ATAAAGCTTTTATCAGACACACTGGTCTC 3′ | 582 | 60 | This study |
MuIFNλ3 | Cloning into pSec Plasmid | F-5′ ACTATGCATCAGAGATGCTCCTCCTGCTGTT 3′ R-5′GATGCTAGCTTATCAGACACACTGGTCTCC 3′ | 582 | 59 | This study |
MuGAPDH | Semi-quantitative PCR | F-5′CGTGCCGCTGGAGAAACC 3′ R-5′TGGAAGAGTGGGAGTTGCTGTTG 3′ | 150 | 60 | This study |
MuMX-1 | Semi-quantitative PCR | F-5′GAAGGCAAGGTCTTGGATG 3′ R-5′GCTGACCTCTGCACTTGACT 3′ | 82 | 60 | This study |
MuISG-15 | Semi-quantitative PCR | F-5′AGCAATGGCCTGGGACCTAAA3′ R-5′AGCCGGCACACCAATCTT 3′ | 124 | 55 | This study |
MuIRF-7 | Semi-quantitative PCR | F-5′CCCAGACTGCCTGTGTAGACG 3′ R-5′CCAGTCTCCAAACAGCACTCG 3′ | 71 | 55 | This study |
MuIL-4 | Semi-quantitative PCR | F-5′GGTCTCAACCCCCAGCTAGT3′ R-5′GCCGATGATCTCTCTCAAGTGAT3′ | 102 | 60 | This study |
MuIL-6 | Semi-quantitative PCR | F-5′TAGTCCTTCCTACCCCAATTTCC3′ R-5′TTGGTCCTTAGCCACTCCTTC3′ | 76 | 60 | This study |
MuIL-10 | Semi-quantitative PCR | F-5′GCTCTTACTGACTGGCATGAG 3′ R-5′CGCAGCTCTAGGAGCATGTG 3′ | 105 | 53 | This study |
Influenza M-gene universal primer | Real Time quantitative PCR | F-5′ATGAGTCTTCTAACCGAGGTCGAAACG 3′ R-5′TGGACAAAGCGTCTACGCTG 3′ | 242 | 60 | (Kuchipudi et al., 2012) |