Fig. 1
Microbiota promotes macrophage recruitment during E. tenella infection. Caecal cells were isolated from 3 weeks old Ross PM3 chickens and stained with Zombie Aqua™, CD45-APC and KUL01-PE. A gate based in FSH-A and SSC-A was placed to remove debris. Live cells were selected using a live/dead marker, Zombie Aqua™. Leukocytes were stained with CD45 antibody and macrophage cells were identified using the KUL01 (MRC1L-B) marker (A). Percentage of KUL01+ cells within the leukocyte population was determined in conventional (blue), GF chickens (red) in non-infected (NI) and at 7 days pi. In green, GF-infected chickens received a microbiota from conventional healthy chickens at 4 days pi (B). Kruskal Wallis test followed by Dunn test. (**P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; NS: non-significant)