Fig. 1

Flagellin in the AIEC strains is elongated depending on the increase in fliC expression. A Morphological changes in non-AIEC K12 and AIEC LF82 strains were visualized using SEM. Each image represents a different bacterial area. Scale bar = 1.0 μm. B Flagellin length per bacterial cell was measured using the ImageJ software. *P < 0.05; P-values were determined using the unpaired Student’s t-test. C Comparison of fliC mRNA expression levels between AIEC and non-AIEC strains clinically isolated from patients with Crohn’s Disease (CD) was performed using quantitative real-time RT-PCR. Data are presented as the mean ± SD. *P < 0.05; P-values were determined using the unpaired Student’s t-test. (n = 10 strains each). D Comparison of fliC mRNA expression levels between the AIEC LF82 and non-AIEC K12 strains. Data are presented as the mean ± SD. *P < 0.05; P-values were determined using the unpaired Student’s t-test. (n = 3 for each strain)